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dc.contributor.authorOchs, Michael F.
dc.contributor.authorLi, Ryan
dc.contributor.authorAhn, Sun Mi
dc.contributor.authorHennessey, Patrick T.
dc.contributor.authorTan, Marietta
dc.contributor.authorSoudry, Ethan
dc.contributor.authorGaykalova, Daria A.
dc.contributor.authorUemura, Mamoru
dc.contributor.authorBrait, Mariana
dc.contributor.authorShao, Chunbo
dc.contributor.authorWestra, William H.
dc.contributor.authorBishop, Justin A.
dc.contributor.authorFertig, Elana J.
dc.contributor.authorCalifano, Joseph A.
dc.date.accessioned2018-04-21T17:45:08Z
dc.date.available2018-04-21T17:45:08Z
dc.date.issued2014
dc.identifier.citationLi, R., Ochs, M. F., Ahn, S. M., Hennessey, P., Tan, M., Soudry, E., & ... Califano, J. A. (2014). Expression Microarray Analysis Reveals Alternative Splicing of LAMA3 and DST Genes in Head and Neck Squamous Cell Carcinoma. Plos ONE, 9(3), 1-10en_US
dc.identifier.urihttps://dx.doi.org/10.1371/journal.pone.0091263
dc.description.abstractPurpose Prior studies have demonstrated tumor-specific alternative splicing events in various solid tumor types. The role of alternative splicing in the development and progression of head and neck squamous cell carcinoma (HNSCC) is unclear. Our study queried exon-level expression to implicate splice variants in HNSCC tumors. Experimental Design We performed a comparative genome-wide analysis of 44 HNSCC tumors and 25 uvulopalatopharyngoplasty (UPPP) tissue samples at an exon expression level. In our comparison we ranked genes based upon a novel score—the Maximum-Minimum Exon Score (MMES) – designed to predict the likelihood of an alternative splicing event occurring. We validated predicted alternative splicing events using quantitative RT-PCR on an independent cohort. Results After MMES scoring of 17,422 genes, the top 900 genes with the highest scores underwent additional manual inspection of expression patterns in a graphical analysis. The genes LAMA3, DST, VEGFC, SDHA, RASIP1, and TP63 were selected for further validation studies because of a high frequency of alternative splicing suggested in our graphical analysis, and literature review showing their biological relevance and known splicing patterns. We confirmed TP63 as having dominant expression of the short DeltaNp63 isoform in HNSCC tumor samples, consistent with prior reports. Two of the six genes (LAMA3 and DST) validated by quantitative RT-PCR for tumor-specific alternative splicing events (Student's t test, P<0.001). Conclusion Alternative splicing events of oncologically relevant proteins occur in HNSCC. The number of genes expressing tumor-specific splice variants needs further elucidation, as does the functional significance of selective isoform expression.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Science
dc.titleExpression Microarray Analysis Reveals Alternative Splicing of LAMA3 and DST Genes in Head and Neck Squamous Cell Carcinomaen_US
dc.typeArticleen_US
dc.typeTexten_US
prism.publicationNamePLOS ONE
prism.volume9
prism.issueIdentifier3
prism.publicationDate2014
dc.identifier.handlehttps://dr.tcnj.edu/handle/2900/2285


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