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dc.contributor.authorOchs, Michael F.
dc.contributor.authorFertig, Elana J.
dc.contributor.authorRen, Qing
dc.contributor.authorCheng, Haxia
dc.contributor.authorHatakeyama, Hiromitsu
dc.contributor.authorDicker, Adam P.
dc.contributor.authorRodeck, Ulrich
dc.contributor.authorConsidine, Michael
dc.contributor.authorChung, Christine H.
dc.date.accessioned2018-04-24T23:14:14Z
dc.date.available2018-04-24T23:14:14Z
dc.date.issued2012
dc.identifier.citationCheng, H., Dicker, A., Fertig, E. J., Cheng, H., Considine, M., Ochs, M. F., & ... Hatakeyama, H. (n.d). Gene expression signatures modulated by epidermal growth factor receptor activation and their relationship to cetuximab resistance in head and neck squamous cell carcinoma. Bmc Genomics, 13en_US
dc.identifier.urihttps://dx.doi.org/10.1186/1471-2164-13-160
dc.description.abstractBackground Aberrant activation of signaling pathways downstream of epidermal growth factor receptor (EGFR) has been hypothesized to be one of the mechanisms of cetuximab (a monoclonal antibody against EGFR) resistance in head and neck squamous cell carcinoma (HNSCC). To infer relevant and specific pathway activation downstream of EGFR from gene expression in HNSCC, we generated gene expression signatures using immortalized keratinocytes (HaCaT) subjected to ligand stimulation and transfected with EGFR, RELA/p65, or HRASVal12D. Results The gene expression patterns that distinguished the HaCaT variants and conditions were inferred using the Markov chain Monte Carlo (MCMC) matrix factorization algorithm Coordinated Gene Activity in Pattern Sets (CoGAPS). This approach inferred gene expression signatures with greater relevance to cell signaling pathway activation than the expression signatures inferred with standard linear models. Furthermore, the pathway signature generated using HaCaT-HRASVal12D further associated with the cetuximab treatment response in isogenic cetuximab-sensitive (UMSCC1) and -resistant (1CC8) cell lines. Conclusions Our data suggest that the CoGAPS algorithm can generate gene expression signatures that are pertinent to downstream effects of receptor signaling pathway activation and potentially be useful in modeling resistance mechanisms to targeted therapies.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.titleGene expression signatures modulated by epidermal growth factor receptor activation and their relationship to cetuximab resistance in head and neck squamous cell carcinomaen_US
dc.typeArticleen_US
dc.typeTexten_US
prism.publicationNameBMC Genomics
prism.volume13
prism.issueIdentifier160
prism.publicationDate2012
dc.identifier.handlehttps://dr.tcnj.edu/handle/2900/2307


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