Adapting the commercial Griess assay to a paper-based microfluidic device

Abstract

Nitric oxide (NO) is an important physiological mediator of the cardiovascular, immune, and nervous systems. The Griess Assay is a cost efficient, colorimetric assay for the indirect measurement of NO concentration in a solution via nitrite. It works by taking nitrite, a breakdown product of NO, and reacting it with sulfanilamide and N-(1-Naphthyl)ethylenediamine (NED) to create a red pink azo dye which can then be evaluated using a RGB detector to create calibration curves. The goal of this project is to convert the Griess assay to a paper-based microfluidic device, which can provide cheap, real-time analysis. Paper analytical methods are: Affordable, Sensitive, Specific, User friendly, Rapid and robust, Equipment free, Deliverable (ASSURED) to end users.