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dc.contributor.authorChen, Xi Jun
dc.contributor.authorSchramm, Joseph
dc.contributor.authorTuohy, Christopher
dc.contributor.authorSkiff, Heather
dc.contributor.authorHummel, Kathleen
dc.contributor.authorSzklarski, Anne R.
dc.contributor.authorVacirca, Nicholas
dc.contributor.authorWolf, Bruce A.
dc.contributor.authorHirsh, Donald J.
dc.identifier.citationChen, X.J.; Schramm, J.; Tuohy, C.; Skiff, H.; Hummel, K.; Szklarski, A.R.; Vacirca, N.; Wolf, B.A.; Hirsh, D.J., "Progress towards a DNA-based model system for the study of electron spin–spin interactions," Biophysical Chemistry, 2007, 129, 2-3, 148-162en_US
dc.descriptionFile not available for download due to copyright restrictionsen_US
dc.description.abstractA DNA-based model system is described for studying electron spin–spin interactions between a paramagnetic metal ion and a nitroxide spin label. The modified base deoxythymidine-EDTA (dT-EDTA) chelates the divalent or trivalent metal ion and produces a new feature in the circular dichroism (CD) spectra that makes it possible to monitor local DNA melting. Based on the results of optical and electron paramagnetic resonance (EPR) experiments, we find that the terminus of the DNA duplex that incorporates dT-EDTA and the spin-label melts at a higher temperature than the rest of the DNA duplex. EPR microwave progressive power saturation experiments performed at 77 K are consistent with the specific binding of Dy(III) at the EDTA site and an intramolecular dipole–dipole interaction between the nitroxide spin-label and the chelated Dy(III). This model system should be suitable for studying the relaxation properties of metal ions by saturation-recovery EPR.en_US
dc.description.sponsorshipAmerican Chemical Society Petroleum Research Funden_US
dc.subjectDNA meltingen_US
dc.subjectCircular dichroismen_US
dc.subjectElectron paramagnetic resonanceen_US
dc.subjectDipole–dipole interactionen_US
dc.subjectCorrelation timesen_US
dc.titleProgress towards a DNA-based model system for the study of electron spin–spin interactionsen_US
prism.publicationNameBiophysical Chemistryen_US

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