• Login
    View Item 
    •   Digital Repository Home
    • TCNJ Scholars (Faculty and Student Research)
    • Faculty Research
    • Chemistry Department
    • View Item
    •   Digital Repository Home
    • TCNJ Scholars (Faculty and Student Research)
    • Faculty Research
    • Chemistry Department
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Structure and dynamics of a DNA-based model system for the study of electron spin–spin interactions

    Thumbnail
    Date
    2009-03
    Author
    Biczo, Ryan
    Hirsh, Donald J.
    Metadata
    Show full item record
    Abstract
    Abstract
    We report on the structure and dynamics of a model system for measuring long-range distances in biological macromolecules by saturation-recovery EPR. Four DNA duplexes that incorporate a paramagnetic dysprosium ion (Dy(III)) and a nitroxide spin–label were examined by electron paramagnetic resonance (EPR), circular dichroism (CD), and ultra-violet absorbance (UV) spectroscopy. Dy(III) is chelated by the modified base deoxythymidine-EDTA, (dT-EDTA). Electron spin–spin interactions between the Dy(III) ion and the nitroxide radical are observed at distances as great as ∼5.3 nm. A slight change in the conformation of those nucleotides lying between the EDTA(Dy(III)) complex and the nitroxide spin–label results in a “stiffening” of the DNA helix on the EPR time scale. Changes in conformation and helix dynamics are due to the binding of the EDTA(Dy(III)) complex to the phosphodiester backbone of the complementary strand. Molecular mechanics calculations indicate that binding occurs in the 5′ direction on the complementary strand, at a position 3 or 4 phosphates distant from the dT-EDTA(Dy(III)) * dA base pair.
    Citation:
    Biczo, R.; Hirsch, D.J., "Structure and dynamics of a DNA-based model system for the study of electron spin–spin interactions," Journal of Inorganic Biochemistry, Volume 103, Issue 3, March 2009, Pages 362-372
    Description
    File not available for download due to copyright restrictions
    URI
    http://dx.doi.org/10.1016/j.jinorgbio.2008.11.013
    Collections
    • Chemistry Department

    DSpace software copyright © 2002-2016  DuraSpace
    | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    DSpace software copyright © 2002-2016  DuraSpace
    | Send Feedback
    Theme by 
    Atmire NV