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dc.contributor.authorBiczo, Ryan
dc.contributor.authorHirsh, Donald J.
dc.date.accessioned2015-06-24T15:30:59Z
dc.date.available2015-06-24T15:30:59Z
dc.date.issued2009-03
dc.identifier.citationBiczo, R.; Hirsch, D.J., "Structure and dynamics of a DNA-based model system for the study of electron spin–spin interactions," Journal of Inorganic Biochemistry, Volume 103, Issue 3, March 2009, Pages 362-372en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.jinorgbio.2008.11.013
dc.descriptionFile not available for download due to copyright restrictionsen_US
dc.description.abstractWe report on the structure and dynamics of a model system for measuring long-range distances in biological macromolecules by saturation-recovery EPR. Four DNA duplexes that incorporate a paramagnetic dysprosium ion (Dy(III)) and a nitroxide spin–label were examined by electron paramagnetic resonance (EPR), circular dichroism (CD), and ultra-violet absorbance (UV) spectroscopy. Dy(III) is chelated by the modified base deoxythymidine-EDTA, (dT-EDTA). Electron spin–spin interactions between the Dy(III) ion and the nitroxide radical are observed at distances as great as ∼5.3 nm. A slight change in the conformation of those nucleotides lying between the EDTA(Dy(III)) complex and the nitroxide spin–label results in a “stiffening” of the DNA helix on the EPR time scale. Changes in conformation and helix dynamics are due to the binding of the EDTA(Dy(III)) complex to the phosphodiester backbone of the complementary strand. Molecular mechanics calculations indicate that binding occurs in the 5′ direction on the complementary strand, at a position 3 or 4 phosphates distant from the dT-EDTA(Dy(III)) * dA base pair.en_US
dc.description.sponsorshipAmerican Chemical Society Petroleum Research Funden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectElectron paramagnetic resonanceen_US
dc.subjectCircular dichroismen_US
dc.subjectLanthanidesen_US
dc.subjectSpin–labelen_US
dc.subjectDNAen_US
dc.titleStructure and dynamics of a DNA-based model system for the study of electron spin–spin interactionsen_US
dc.typeArticleen_US
dc.typeTexten_US
prism.publicationNameJournal of Inorganic Biochemistryen_US
prism.volume103
prism.issueIdentifier3
prism.startingPage362
prism.endingPage372
dc.identifier.handlehttps://dr.tcnj.edu/handle/2900/90


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