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    Optimizing YtfE MALDI TOF MS ion intensity by matrix selection and sample deposition

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    Poster (548.5Kb)
    Date
    2022
    Author
    Martinez, Justin
    Alpizar, Cristian Herrera
    Peddinti, Sri Manyata
    Ekanger, Levi
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    Abstract
    Abstract
    Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF MS) is a technique used to analyze the mass of biomolecules including proteins. Sample preparation for MALDI TOF MS requires a matrix (a small organic compound) which absorbs and transfers energy from a laser pulse to the sample analyte. Energy transfer from matrix causes analyte desorption from the sample plate into the gas phase where desolvation and ionization occurs. The mass-to-charge ratio (m/z) of ionized analyte is detected by the mass analyzer. MALDI TOF MS signal intensity can be optimized by through matrix selection, pH, and sample deposition methods. Optimizing the intensity of YtfE ions is part of our broader objective to discover small molecule inhibitors of YtfE to combat pathogenic bacterial infections.
    Description
    Department of Chemistry
    Rights
    File access restricted due to FERPA regulations
    URI
    http://dr.tcnj.edu/handle/2900/4129
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    • MUSE (Mentored Undergraduate Summer Experience)

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